The glucocorticoid receptor

The glucocorticoid receptor.

The glucocorticoid receptor resource

The glucocorticoid receptor resource focuses on the structure-function relationships of the glucocorticoid receptor. As well as links to sequence and bibliographic databases via the World Wide Web, the database contains sequence comparisons of receptors from different species and source information for glucocorticoid receptor clones, probes, cell lines and antibodies. The resource allows the el...

The Purified Activated Glucocorticoid Receptor

The structure of purified preparations of activated (DNA-binding) glucocorticoid receptor (GR) was analyzed in the presence or absence of DNA. A 35-base pair DNA fragment harboring a strong GR-binding site from the mouse mammary tumor virus promoter (-189/-166) was used for stoichiometric analysis of the GR . DNA complex. Glycerol gradient centrifugation was utilized in order to separate the 6 ...

Non-glucocorticoid receptor-mediated effects of the potent glucocorticoid deacylcortivazol.

Deacylcortivazol (DAC), a potent glucocorticoid, contains a phenyl-pyrazole moiety fused to the 2--3 position of the traditional steroid nucleus. When incubated with glucocorticoid-resistant mutants derived from the glucocorticoid-sensitive human leukemic cell line CEM-C7, DAC caused significant growth inhibition. However, this effect required 1 microM DAC, a concentration 50 times higher than ...

Characterization of Glucocorticoid Receptors and Glucocorticoid Receptor mRNA in Human Leukemia Cells : Stabilization of the Receptor

by the action of a serine protease. M 52,000-receptor fragments were present in cytosol from 24 of 52 leukemia cell samples. Only normal size glucocorticoid receptors were present in cytosol samples if diisopropylfiuorophosphate (DFP). a potent inhibitor of serine proteases. was added to the hypotonic buffer used for cytosol preparation. Receptor proteolysis was not inhibited by hydrolyzed DFP....